how long does it take to od

Normally OD comes to 0.6 in 1-2 hours, but in my case it takes 5-6.. To get a starting OD of 0.1, 2 (OD) / 25 (OD) (ml) = 0.08ml. This makes me think that your overnight culture's OD must be really low because if it were around 2 then inoculating 10.

Normally OD comes to 0.6 in 1-2 hours, but in my case it takes 5-6.
Normally OD comes to 0.6 in 1-2 hours, but in my case it takes 5-6. from qph.fs.quoracdn.net

What you really want to know. What you really want to know from an OD600 reading is the density of the cells e.g. in cells/mL. And to get this you need a standard curve. In other.

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